Contextualizing Genes by Using Text-Mined Co-Occurrence Features for Cancer Gene Panel Discovery.

Developing a biomedical-explainable and validatable text mining pipeline can help in cancer gene panel discovery. We create a pipeline that can contextualize genes by using text-mined co-occurrence features. We apply Biomedical Natural Language Processing (BioNLP) techniques for literature mining in the cancer gene panel. A literature-derived 4,679 × 4,630 gene term-feature matrix was built. The EGFR L858R and T790M, and BRAF V600E genetic variants are important mutation term features in text mining and are frequently mutated in cancer. We validate the cancer gene panel by the mutational landscape of different cancer types. The cosine similarity of gene frequency between text mining and a statistical result from clinical sequencing data is 80.8%. In different machine learning models, the best accuracy for the prediction of two different gene panels, including MSK-IMPACT (Memorial Sloan Kettering-Integrated Mutation Profiling of Actionable Cancer Targets), and Oncomine cancer gene panel, is 0.959, and 0.989, respectively. The receiver operating characteristic (ROC) curve analysis confirmed that the neural net model has a better prediction performance (Area under the ROC curve (AUC) = 0.992). The use of text-mined co-occurrence features can contextualize each gene. We believe the approach is to evaluate several existing gene panels, and show that we can use part of the gene panel set to predict the remaining genes for cancer discovery.

Comprehensive assessments of germline deletion structural variants reveal the association between prognostic MUC4 and CEP72 deletions and immune response gene expression in colorectal cancer patients.

BACKGROUND: Functional disruptions by large germline genomic structural variants in susceptible genes are known risks for cancer. We used deletion structural variants (DSVs) generated from germline whole-genome sequencing (WGS) and DSV immune-related association tumor microenvironment (TME) to predict cancer risk and prognosis. METHODS: We investigated the contribution of germline DSVs to cancer susceptibility and prognosis by silicon and causal inference models. DSVs in germline WGS data were generated from the blood samples of 192 cancer and 499 non-cancer subjects. Clinical information, including family cancer history (FCH), was obtained from the National Cheng Kung University Hospital and Taiwan Biobank. Ninety-nine colorectal cancer (CRC) patients had immune response gene expression data. We used joint calling tools and an attention-weighted model to build the cancer risk predictive model and identify DSVs in familial cancer. The survival support vector machine (survival-SVM) was used to select prognostic DSVs. RESULTS: We identified 671 DSVs that could predict cancer risk. The area under the curve (AUC) of the receiver operating characteristic curve (ROC) of the attention-weighted model was 0.71. The 3 most frequent DSV genes observed in cancer patients were identified as ADCY9, AURKAPS1, and RAB3GAP2 (p < 0.05). The DSVs in SGSM2 and LHFPL3 were relevant to colorectal cancer. We found a higher incidence of FCH in cancer patients than in non-cancer subjects (p < 0.05). SMYD3 and NKD2DSV genes were associated with cancer patients with FCH (p < 0.05). We identified 65 immune-associated DSV markers for assessing cancer prognosis (p < 0.05). The functional protein of MUC4 DSV gene interacted with MAGE1 expression, according to the STRING database. The causal inference model showed that deleting the CEP72 DSV gene affect the recurrence-free survival (RFS) of IFIT1 expression. CONCLUSIONS: We established an explainable attention-weighted model for cancer risk prediction and used the survival-SVM for prognostic stratification by using germline DSVs and immune gene expression datasets. Comprehensive assessments of germline DSVs can predict the cancer risk and clinical outcome of colon cancer patients.